Table 2.
Spectrum of Rifr mutants generated during outgrowth and subsequent cell division of spores of different B. subtilis strains
| Base substitutions | Rifr strains | |||||||
|---|---|---|---|---|---|---|---|---|
| Wild type | disA | mfd | disA mfd | |||||
| Frequencya | Position of mutationb | Frequencya | Position of mutationb | Frequencya | Position of mutationb | Frequencya | Position of mutationb | |
| A→T | 1/20 | (1445)/1 | 1/20 | (1372)/1 | ||||
| T→A | 1/20 | (1374)/1 | ||||||
| A→G | 8/20 |
(1379)/2 (1406)/3 (1445)/3 |
8/20 |
(1406)/6 (1445)/2 |
2/20 |
(1406)/1 (2033)/1 |
3/20 |
(1406)/2 (1445)/1 |
| G→A | 2/20 |
(1454)/1 (1956)/1 |
2/20 |
(1462)/1 (1959)/1 |
||||
| T→G | 1/20 | (2028)/1 | 3/20 |
(1386)/1 (2013)/1 (2021)/1 |
1/20 | (1543)/1 | ||
| G→T | 4/20 |
(1391)/1 (1414)/2 (2011)/1 |
1/20 | (1428)/1 | ||||
| G→C | ||||||||
| C→G | 1/20 | (1367)/1 | 3/20 |
(1444)/2 (2015)/1 |
||||
| T→C | 1/20 | (2042)/1 | 1/20 | (2040)/1 | ||||
| C→T | 10/20 | (1444)/10 | 1/20 | (1444)/1 | 1/20 | (1460)/1 | 7/20 |
(1383)/1 (1433)/1 (1444)/3 (1460)/1 (2034)/1 |
| A→C | 2/20 | (1445)/2 | ||||||
| C→A | 2/20 |
(1405)/1 (1957)/1 |
7/20 |
(1405)/3 (1433)/4 |
2/20 |
(1405)/1 (1432)/1 |
||
a
Frequency→Number of clones with the specific base substitution/number of clones sequenced.
b
Position of mutation→Position of mutation in rpoB/number of clones with that mutation.