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. 2018 Jul 25;49(5):481–497. doi: 10.1007/s10735-018-9787-y

Table 2.

Primary antibodies and their dilutions used in immunohistochemical analyses

Primary antibodies Source and concentration Dilution cryostat sections Dilution paraffin sections
Cathepsin B (mouse anti-human) Faculty of Pharmacy, University of Ljubljana (3E1); 0.918 mg/mL 1:200 1:100
Cathepsin K (rabbit anti-human) Abcam (ab19027); 0.5 mg/mL 1:200 1:200
Cathepsin X (goat anti-human) R&D systems (AF934); 0.5 mg/mL 1:200 1:200
CD133 (prominin-1) (mouse anti-human) Miltenyi Biotech (W6B3C1); 0.1 mg/mL 1:50 1:10
SDF-1α (stromal-derived factor 1α) (rabbit anti-human) Abcam (ab9797); 0.5 mg/mL 1:200 1:200
SMA (smooth muscle actin) (rabbit anti-human) Abcam (ab5694); 0.2 mg/mL 1:200 1:200
CD68 (mouse anti-human) Dako (clone EBM 11); 0.46 mg/mL / 1:50