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Mechanical tool aligned 0° data. Sedimentation coefficient distribution plot for 0.7 mg/mL bovine serum albumin in phosphate–chloride buffer (pH 6.8, I = 0.1). Alignment with Nanolytics mechanical tool set for 0°. I: 1st run. II: 2nd run after roller re-dispersal. III. 3rd run at 0° after completion of all the misalignment and re-dispersal experiments. IV: Enhanced manual alignment, 0°
