Table 2.
HER2 status determined by IHC, FISH, and ddPCR
| Case no. | IHC | HER2/CEP17 ratio (FISH) | HER2/EIF2C1 ratio Triplicate (ddPCR) |
HER2/EIF2C1 ratio Mean ± SD (ddPCR) |
||
|---|---|---|---|---|---|---|
| B1 | ND | Amp | 9.0 | 8.6 | 8.8 | 8.8 ± 0.20 |
| B2 | ND | 1.0 | 1.0 | 1.0 | 0.9 | 1.0 ± 0.06 |
| B4 | 3 + | Amp | 8.4 | 7.9 | 7.7 | 8.0 ± 0.36 |
| B5 | 3 + | 0.8 | 0.9 | 0.9 | 1.2 | 1.0 ± 0.17 |
| B7 | ND | 1.1 | 0.9 | 0.9 | 0.9 | 0.9 ± 0.00 |
| B8 | 1 + | 1.1 | 1.0 | 1.0 | 0.9 | 1.0 ± 0.06 |
| B9 | ND | 1.8 | 0.6 | 0.6 | 0.7 | 0.6 ± 0.06 |
| B11 | 3 + | Amp | 6.1 | 6.0 | 5.6 | 5.9 ± 0.26 |
| B13 | 1 + | 1.1 | 1.3 | 1.3 | 1.2 | 1.3 ± 0.06 |
| B14 | 3 + | Amp | 4.2 | 4.1 | 4.2 | 4.2 ± 0.06 |
| B15 | 2 + | 1.1 | 1.2 | 1.2 | 1.0 | 1.1 ± 0.12 |
| B16 | 2 + | 1.1 | 3.6 | 3.6 | 3.6 | 3.6 ± 0.00 |
| B18 | 1 + | 1.0 | 1.1 | 1.1 | 1.1 | 1.1 ± 0.00 |
| B19 | 3 + | Amp | 18.5 | 16.3 | 17.2 | 17.3 ± 1.11 |
| B21 | 0 | 1.9 | 1.4 | 1.3 | 1.4 | 1.4 ± 0.06 |
| B23 | 1 + | 1.0 | 1.0 | 1.0 | 1.1 | 1.0 ± 0.06 |
| B24 | 2 + | 1.0 | 1.9 | 2.0 | 2.3 | 2.1 ± 0.21 |
| B25 | ND | 1.7 | 1.1 | 1.2 | 1.2 | 1.2 ± 0.06 |
| B26 | ND | 1.5 | 1.3 | 1.3 | 1.5 | 1.4 ± 0.12 |
| B28 | 3 + | Amp | 6.9 | 6.6 | 6.9 | 6.8 ± 0.17 |
| B29 | ND | Amp | 6.3 | 6.5 | 6.2 | 6.3 ± 0.15 |
| B32 | ND | Amp | 2.7 | 2.5 | 2.6 | 2.6 ± 0.10 |
| B35 | ND | 1.1 | 1.0 | 0.9 | 0.9 | 0.9 ± 0.06 |
| B36 | ND | 1.1 | 1.0 | 1.0 | 1.0 | 1.0 ± 0.00 |
| B37 | ND | Amp | 1.3 | 1.3 | 1.3 | 1.3 ± 0.00 |
| B38 | ND | 1.6 | 1.1 | 1.05 | 1.1 | 1.1 ± 0.30 |
| B40 | ND | 1.0 | 0.7 | 0.7 | 0.7 | 0.7 ± 0.00 |
| B41 | ND | 1.0 | 1.3 | 1.2 | 1.2 | 1.2 ± 0.06 |
| B43 | 3 + | Amp | 14.1 | 13.8 | 14.2 | 14.0 ± 0.21 |
| B48 | ND | 1.0 | 6.7 | 7.2 | 7.0 | 7.0 ± 0.25 |
| B62 | 1 + | 1.0 | 1.1 | 1.1 | 1.1 | 1.1 ± 0.00 |
Amp HER2 amplification as cluster, ND not done