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. 2018 Aug 9;61(11):2333–2343. doi: 10.1007/s00125-018-4705-0

Fig. 3.

Fig. 3

TLR9-deficiency does not protect from beta cell death induced by STZ. (a) Incidence of diabetes development induced by high-dose STZ (100 mg/kg, two i.p. injections) in 5–6-week-old female Tlr9−/− NOD mice (black circles; n = 5 mice) and Tlr9+/+ NOD littermates (white circles; n = 5 mice). (b) Incidence of diabetes development induced by low-dose STZ (40 mg/kg, five i.p. injections) in 5–6-week-old female Tlr9−/− NOD mice (black circles; n = 7 mice) and Tlr9+/+ NOD littermates (white circles; n = 7 mice). (c) Apoptosis of islet cells in response to STZ (40 ng/ml) insult in vitro determined by Annexin V and 7-AAD staining (n = 4 mice per group). White circles, live cells (Annexin V−/7-AAD−); grey triangles, early apoptotic cells (Annexin V+/7-AAD−); inverted white triangles, late apoptotic cells (Annexin V−/7-AAD+); black circles, dead cells (Annexin V+/7-AAD+). All experiments were performed once. Data in (c) are expressed as means (SD). Data in (a, b) were analysed by logrank test for survival and in (c) by two-tailed unpaired Student’s t test (NS for all)