Fig. 2.

Confirmation of CAR-SIV isoform expression in human islets. qRT-PCR analysis of CXADR isoform expression in (a) isolated human islets (n = 5 individuals) and (b) LCM human islets (n = 2 individuals) demonstrates that the SIV and TVV isoforms are highly expressed, CAR4/7 is present at low levels, and CAR3/7 and CAR2/7 are barely detectable. Data were normalised to the relative expression of three housekeeping genes, β-actin, GAPDH and B2M. Relative expression is presented as the mean ± SEM. (c) RNAseq data showing CAR isoform expression in islets from five normoglycaemic individuals (mean ± SEM) [23]. RPKM, reads per kilobase of exon model per million mapped reads. (d) Confirmation of CAR-SIV protein expression in isolated human islets and EndoC-βH1 cells as assessed by western blotting using CAR-CT and CAR-ECD antisera and loading control glyceraldehyde 3-phosphate dehydrogenase (GAPDH). H., human