Fig. 4.

Equilibrium distributions of organelle marker proteins and ACSL3 in MCF-7 subcellular fractions. Equal volume samples from each MCF-7 sucrose gradient fraction were subjected to SDS-PAGE separation and immunoblotted for ACSL3 using two different antibodies, one raised against a C-terminal epitope and the other against an N-terminal epitope of the enzyme. The gradient distributions of a panel of organelle marker proteins was similarly determined by immunoblotting. Data presented are representitive of experiments repeated 2–3 times with similar results