Fig. 1.

MAGEC2 interacts with TRIM28 in melanoma cell lines. a Endogenous MAGEC2 binds with TRIM28 in A375 cells. A375 cells (1 × 10⁸) were lysed and immunoprecipitated with mIgG, anti-MAGEC2 or anti-TRIM28 antibodies and immunoblotted with anti-MAGEC2 or anti-TRIM28 antibodies. Mouse IgG (mIgG) was used as a negative control for the immunoprecipitation. b Endogenous MAGEC2 binds with TRIM28 in Hs 695 T cells. Hs 695 T cells (1 × 10⁸) were lysed and immunoprecipitated with anti-mIgG or anti-TRIM28 antibodies and immunoblotted with anti-MAGEC2 or anti-TRIM28 antibodies. Mouse IgG (mIgG) was used as a negative control for the immunoprecipitation. The experiments were repeated at least three times