Table 2.
Characteristics of the 20 polymorphic SSR markers developed for genetic diversity analyses of P. edulis
| Primer / locus name | GenBank ANSSa | SSR motif (5′ to 3′) | forward primer (5′ to 3′) | reverse primer (5′ to 3′) | Expected allele size (bp) | Observed allele size range (bp) | Ta (°C) |
|---|---|---|---|---|---|---|---|
| PE_01 | GB|JZ730850.1 | (TC)9 | TCACCGCAGTTTTCAGTCTCTA | ATGAGATTCGCCATAGGTTTGT | 102 | 122–146 | 59 |
| PE_02 | GB|JZ730431.1 | (AAG)6 | TGAATCTGCAAAGACATCTGCT | AACTGAGACAACTCCATTGACG | 114 | 108–126 | 57 |
| PE_03 | GB|JZ730426.1 | (TA)10 | GAGACGACGACCAATGTTGTTA | CTCTCTATCACTCCTGACGGCT | 125 | 106–142 | 56 |
| PE_04 | GB|JZ732709.1 | (ACACCC)5 | GGGGAATTAGAGATGGAAAGATAGA | TTGAGGGTGTGAAGTGGTACAG | 131 | 137–143 | 57 |
| PE_05 | GB|JZ732789.1 | (TG)10 | GCCGTATCTCCATTTGTTGATT | TCCATGCTCCTCACACATTATC | 141 | 140–168 | 57 |
| PE_06 | GB|JZ731502.1 | (AC)6 | GCTTACGCCAAGAACTGAAACT | AATAGCAATCTCTTTCCCTCCC | 164 | 162–182 | 55 |
| PE_07 | GB|JZ730947.1 | (CTC)4 | GTTGGACGACTGGGTTTTATGT | GAATGACGCTAGTTTGCTGTTG | 314 | 319–340 | 55 |
| PE_08 | GB|JZ731328.1 | (GCT)6 | CCAACAGCAATCCATATTACCA | ATTTCTCAAGTCAGTCCGAGGT | 172 | 162–189 | 57 |
| PE_09 | GB|JZ732633.1 | (CAG)6 | AACCAAATGACAGGAGCATCTT | CAATTTCTTCATACTGGGTGGC | 184 | 180–206 | 55 |
| PE_10 | GB|JZ730151.1 | (CTT)5 | GGGTAACGATTTGAATAATGCG | GTGAAGCGGGATCTACACTGA | 188 | 176–200 | 52 |
| PE_11 | GB|JZ730075.1 | (AGAGA)4 | GTCAGCCTTTCTCTCTCGTCTC | AGGGGAGTGTGTTATCAAATGG | 223 | 249–264 | 56 |
| PE_12 | GB|JZ730223.1 | (AGC)6 | GACCATCGGTAAGGAGAACTTG | GGATATGAGCTGGATAGCTGGT | 226 | 213–243 | 55 |
| PE_13 | GB|JZ731331.1 | (CAC)5 | GCAAAAGTTCTACCAGCGTTTC | GGTTTGTTGATCCCAACGTAAT | 229 | 242–251 | 57 |
| PE_14 | GB|JZ731120.1 | (GAA)10 | AATTACTTTCATGTCCAACGCC | TTTTCATCACTACCATCCCAGTC | 229 | 228–243 | 55 |
| PE_15 | GB|JZ731840.1 | (TGT)5 | AGTTGAGATTGTACTCCACGTTGT | CTACTTCAGTTCCGGCCTCTTA | 260 | 233–260 | 57 |
| PE_16 | GB|JZ732555.1 | (AC)9 | GGCGATATTCAAGAAAGCAACT | TCTTTTCACGCTTCCATCTCTT | 355 | 319–339 | 57 |
| PE_17 | GB|JZ729809.1 | (GA)8 | GGGGCTTTTCTTGTTTGAAGAT | ATTGGAGGCAACTCATCAGAAT | 361 | 370–382 | 57 |
| PE_18 | GB|JZ732589.1 | (GA)9 | ATGAAGAGTGAAGAGGCTGGAG | AGGAGCAAGCAAATAGAAATCG | 306 | 304–322 | 57 |
| PE_19 | GB|JZ732378.1 | (TGC)5 | GTGTACATGCCATAGAATGAGT | GACTTCATCATATACGGCGGTT | 167 | 180–192 | 57 |
| PE_20 | GB|JZ730154.1 | (TGC)5 | TACTTTATGGCAGAGAATGCGA | CTTGAGAGCCCTGAGACTTCAT | 178 | 200–227 | 57 |
aANSS Accession number of source sequences