Fig. 1.

Knockdown of gna1 results in a decrease in secreted hydrolytic enzymes. a Radius of the zone of hydrolysis in carboxymethylcellulose (CMC) medium; b phenotype of strains in microcrystalline cellulose (MCC) medium; c filter-paper-hydrolyzing (FPase) activity; d carboxymethylcellulose (CMCase) activity; e β-glucosidase (BGL) activities; f xylanase activities in different strains with or without 2 mM 8-Br-cAMP. Mycelia were grown in 500-ml Erlenmeyer flasks containing 200 ml MCC medium, with constant shaking at 120 rpm and 28 °C. After 8 days of incubation, the fermentation broth was used as the enzyme source. The statistical significance is indicated by an asterisk (P-value < 0.05 in t-test analysis) or by two asterisks (P-value < 0.01 in t-test analysis). Experiments were carried out in biological triplicates