Fig. 2.

Knockdown of gna1 results in differential expression of cellulase and xylanase genes as well as their transcriptional regulators. a Transcript levels of cellulase and xylanase genes were detected by qRT-PCR in the wild-type strain, pG14 mutant, pGP6 mutant, and pG14 mutant with the addition of 2 mM 8-Br-cAMP. b Transcript levels of transcriptional regulators were detected by qRT-PCR in the wild-type strain, pG14 mutant, pGP6 mutant, and pG14 mutant with the addition of 2 mM 8-Br-cAMP. The transcripts all were normalized against β-actin amplified with primers qActin (s) and qActin (as) (Additional file 1: Table S1). There was a significant difference between the mutant and wild-type strain, as indicated by an asterisk (P-value < 0.05 in t-test analysis) or by two asterisks (P-value < 0.01 in t-test analysis). Experiments were performed in triplicate