Table 1.
Variation of cellulase genes detected by RNA-seq profiling (RPKM > 20)
| Gene number in C. globosum NK102 | GH family/regulators | Protein/gene name predicted by Longoni et al. [19] | C. globosum gene ID (NCBI) | log2Ratio (WT/pG14) | FDR | Up/down-regulation (pG14/WT) |
|---|---|---|---|---|---|---|
| 1 | GH1 | EC 3.2.1.21 β-glucosidase | CHGG_05642 | − 1.3 | 2.32E − 23 | Down |
| 15 | GH3 | EC 3.2.1.37 β-xylosidase | CHGG_06807 | − 1.8 | 2.56E−47 | Down |
| CHGG_01059 | − 1.7 | 5.34E−79 | Down | |||
| CHGG_03421 | − 4.8 | 0 | Down | |||
| 8 | GH5 | EC 3.2.1.4 endoglucanase/EC 3.2.1.78 mannase/egl2 | CHGG_01188 | − 0.6 | 0.000149 | Down |
| 1 | GH45 | EC 3.2.1.4 endoglucanase/egl1 | CHGG_10708 | − 2.2 | 7.68E−20 | Down |
| CHGG_08509 | − 0.4 | 0.231624 | Down | |||
| 7 | GH6 | EC 3.2.1.4 endoglucanase/EC 3.2.1.91 cellobiohydrolase/cel6a | CHGG_10762 | − 2.3 | 1.41E−09 | Down |
| CHGG_06834 | − 1.0 | 0.002945 | Down | |||
| 8 | GH7 | EC 3.2.1.4 endoglucanase/EC 3.2.1.91 exoglucanase/cel7a | CHGG_08330 | − 1.1 | 0.003731 | Down |
| CHGG_08475 | − 0.5 | 0.011536 | Down | |||
| 30 | GH61 | EC 3.2.1.4 endoglucanase/copper-dependent polysaccharide monooxygenases | CHGG_03415 | − 2.7 | 2.13E−09 | Down |
| CHGG_06059 | − 1.2 | 8.34E−10 | Down | |||
| CHGG_08275 | − 2.3 | 3.67E−31 | Down | |||
| CHGG_04473 | − 0.5 | 0.078539 | Down | |||
| CHGG_00362 | − 1.9 | 9.65E−09 | Down | |||
| CHGG_07756 | − 1.8 | 1.02E−16 | Down | |||
| CHGG_07451 | − 3.4 | 1.60E−23 | Down | |||
| CHGG_00683 | − 1.4 | 1.01E−07 | Down | |||
| CHGG_07676 | − 2.1 | 4.92E−05 | Down | |||
| CHGG_06144 | − 2.1 | 4.92E−05 | Down | |||
| 6 | GH10 | EC 3.2.1.8 endo-1,4-xylanase/xln | CHGG_00030 | − 0.9 | 0.077566 | Down |
| CHGG_00304 | − 1.0 | 0.034787 | Down | |||
| 11 | CDH | EC 1.1.9978 cellobiose dehydrogenase | CHGG_03380 | − 1.8 | 0 | Down |
| Negative regulators | AceI | ace1 | CHGG_00776 | 0.5 | 3.78E−11 | Up |
| CreA | cre1 | CHGG_03907 | < − 0.1 | 0.601103 | – | |
| Positive regulators | XlnR | xyr1 | CHGG_03981 | Not detected | ||
| AceII | ace2 | – | ||||
| Hap2 | hap2 | CHGG_05974 | − 0.7 | 4.99E−10 | Down | |
| Hap3 | hap3 | CHGG_01529 | − 1.8 | 1.57E−13 | Down | |
| Hap5 | hap5 | CHGG_03369 | − 1.6 | 3.19E−19 | Down | |
| Clr1 | clr1 | CHGG_04631 | − 3.1 | 4.29E−58 | Down | |
| Clr2 | clr2 | CHGG_04595 | − 2.3 | 3.25E−09 | Down | |
| Photoreceptor and light related regulators | LaeA | LaeA | CHGG_01690 | − 1.7 | 0.000133 | Down |
| VeA | VeA | CHGG_10370 | − 0.5 | 2.25E−13 | Down | |
| VelB | VelB | CHGG_09077 | − 1.7 | 1.37E−42 | Down | |
| ENV1 | env1 | CHGG_00392 | 1.5 | 1.05E−12 | Up |