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. 2018 Oct 12;10:45. doi: 10.1186/s13099-018-0272-7

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Composite microscopy image of purified oocysts before (a) and after (b) 15 cycles of freeze and thaw. Black arrows = intact oocysts; black arrowheads = oocysts, partially or completely empty; white arrows = sporocysts; white arrowhead = empty sporocyst. Scale bar = 10 μm