Table 1.
List of primers used for RT-qPCR and Tg26 mouse genotyping
| Gene target | Direction | Sequence (5′ to 3′) |
|---|---|---|
| Partial Gag (p17) | T760—forward | GGATAGATGTAAAAGACACCA |
| T946—reverse | ACCTGGCTGTTGTTTCCTGTGTC | |
| Env (gp120) | T876—forward | CCGAAGGAATAGAAGAAGAAG |
| T691—reverse | AGAGTAAGTCTCTCAAGCGG | |
| Tat2 (spliced variant) | T1002—forward | TGGAAGCATCCAGGAAGTCAGCC |
| T1003—reverse | TTCTTCTTCTATTCCTTCGGGCC | |
| Tat1 (unspliced variant) | T1002—forward | TGGAAGCATCCAGGAAGTCAGCC |
| T1007—reverse | GAGAAGCTTGATGAGTCTGACTG | |
| Nef | F3.3—forward | CCGAAGGAATAGAAGAAGAAG |
| R3.3—reverse | CTTGTAGCACCATCCAAAGG | |
| Vpu | T1002—forward | TGGAAGCATCCAGGAAGTCAGCC |
| R1.3—reverse | GTGGTGGTTGCTTCCTTCC | |
| PCR for mouse genotyping | T361—Tg26 forward | GATCTGTGGATCTACCACACACA |
| T363—Tg26 reverse | GCTGCTTATATGCAGCATCTGAG |