Table 1.
Results from the first sequencing run
| Sample | DIN | #Pre-hyb Cycles | Hyb Input (ng) | #Sequences | Aligned | On target | No PCR Dup | Final | MedianDepth (per million reads) |
|---|---|---|---|---|---|---|---|---|---|
| NA12878 | 9.3 | 8 | 750 | 3,387,238 | 99.37% | 75.07% | 48.16% | 48.14% | 113.07 |
| NA12891 | 9.1 | 8 | 750 | 3,188,127 | 99.48% | 72.49% | 49.56% | 49.54% | 118.25 |
| NA12892 | 9.1 | 8 | 750 | 3,115,861 | 98.88% | 73.80% | 50.41% | 50.40% | 119.07 |
| NT1 | 6.4 | 8 | 401 | 11,305,259 | 99.72% | 71.49% | 31.15% | 31.13% | 63.42 |
| T1 | 2.7 | 10 | 362.5 | 2,866,206 | 99.37% | 72.20% | 35.17% | 35.13% | 66.29 |
| T2 | 2.2 | 10 | 11,9 | 2,813,675 | 97.65% | 81.07% | 4.45% | 4.40% | 4.98 |
| T3 | 3 | 10 | 87 | 2,730,937 | 99.39% | 77.30% | 13.86% | 13.84% | 25.63 |
| T4 | 2 | 10 | 12 | 2,347,778 | 97.87% | 71.73% | 5.09% | 5.05% | 7.24 |
| T5 | 3.6 | 10 | 32.4 | 3,077,225 | 99.35% | 79.63% | 8.45% | 8.42% | 13.65 |
| NA12892 | 9.1 | 10 | 156 | 2,983,904 | 98.63% | 77.31% | 49.21% | 49.19% | 106.24 |
From left to right: sample name, DIN value for input DNA, number of cycles in the pre-hyb PCR, total amount of digested and adaptor-tagged library used in the hybridization step, number of sequences obtained, percentage of sequences aligned to the human reference genome, percentage of sequences on target, percentage of sequences after PCR duplicates removal, percentage of sequences after low-quality-mapped-reads removal, median of the depth in the target regions obtained per million reads (normalized for a more realistic comparison)