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. 2018 Oct 12;17:161. doi: 10.1186/s12934-018-1009-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Separate analysis of the two differences that distinguish pro-α-factor(I) from pro-α-factor. The experiment was performed as in Fig. 2a, except that the signals were not normalized and the Ost1 signal sequence was used together with either pro-α-factor, or pro-α-factor(I), or the MUT1 or MUT2 variant of the α-factor pro region. a.u. arbitrary units