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(a) Genetic organization of cotX2 (BMQ_pBM60028), cotX1 (BMQ_pBM60029), and cotW (BMQ_pBM60030) on plasmid pBM600 of B. megaterium QM B1551. DNA sequence and reverse transcription-PCR (RT-PCR) analyses indicate that cotW is monocistronic, whereas cotX1 and cotX2 are organized as an operon comprising both genes. (b) Clustal Omega sequence alignment of B. subtilis (Bs) CotX with B. megaterium (Bm) CotX1 and CotX2. Asterisks denote conserved residues.
