FIG 4.

Yeast growth inhibitory activity of extract samples from filtrates and resupended OMV. Resuspended OMV and filtrate fractions (300-kDa cutoff) were extracted under identical conditions, dried, and redissolved in equal volumes of ethanol. Ethanol aliquots were added to dilute yeast cultures (S30 strain) in the volumes indicated, and overnight growth was monitored by turbidity at 600 nm (n = 3 ± standard deviation [SD]; some error bars smaller than symbols; P < 0.05 for all samples of >0.5 μl). More details are given in Materials and Methods.