FIG 5.

Specific activity of OMV preparations in yeast growth assays. Two separate preparations of C3 OMV were isolated from culture supernatants grown in MM (blue and violet, “C3 OMV”). Orange data points represent OMV that were heat treated at 70°C before testing for activity. OMV isolated from C3 HSAF⁻ mutant cells grown in MM are shown by squares (HSAF⁻, red-brown). For preparations from 10% TSB, activity curves are shown by green triangles for C3 wild type and brown squares for HSAF⁻ mutant (n = 3 ± SD; some error bars smaller than symbols). The activity of heat-treated C3 OMV from the same 10% TSB preparation is shown at 5 μg total protein as a red circle (n = 3 ± SD; error bars smaller than symbol). No activity was observed in samples of similar volume from the OMV-free supernatants of these preparations. Data are representative of more than 20 preparations of C3 OMV, which all showed full activity at 5 μg of total protein.