FIG 5.

Resuscitation from 2-weeks-nitrogen-starved Synechocystis sp. wild-type and ΔcphA cultures in BG-11 liquid medium and on agar under continuous light. (A and B) Absorbance of phycobiliproteins at 630 nm (A) and absorbance of chlorophyll a/b at 680 nm (B) during regreening. Values shown are the means of three biological replicates. (C) Cultures of resuscitating wild type (WT) and the ΔcphA mutant; 0 h, induction of resuscitation. (D) Growth curve (OD₇₅₀) of wild-type and the ΔcphA mutant cultures during resuscitation. Values shown are the means of three biological replicates. (E) Drop plate method of resuscitating wild-type and ΔcphA mutant cells on BG-11 agar plates containing 17.3 mM nitrate (standard concentration in BG-11 medium). Cultures were adjusted to an OD₇₅₀ of 1.0, diluted in a 10-fold dilution series, and cultivated under continuous light. Photos were taken starting after 2 days of cultivation. Assays were performed using three biological replicates per strain. d, days.