TABLE 4.
Plasmids used in this studya
| Plasmid | Genotype/description | Reference or source |
|---|---|---|
| pX | Vector for the integration of genes into the amyE gene | 99 |
| pChen3 | opuC operon with native promoter cloned into pX-amyE | 82 |
| pHK2 | opuFB.i. operon with native promoter cloned into pX-amyE | This study |
| pHK3 | opuFB.p. operon with native promoter cloned into pX-amyE | This study |
a
All plasmids carried the genes bla, which confers resistance against the antibiotic ampicillin in E. coli but not in B. subtilis, and cat, which confers resistance against the antibiotic chloramphenicol in B. subtilis.