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. 2018 Sep 7;233(5):592–606. doi: 10.1111/joa.12880

Figure 5.

Figure 5

E7.5 transplanted embryos have a predominantly dorsal localisation of GFP + cells to the spinal cord white matter and spread through the injury site. (A, B) Fluorescent stereoscopic examination of embryos harvested at E7.5 revealed extensive cell spread from the transplantation site. (C) In transverse sections GFP + cells were distributed in a ‘halo’ within the spinal cord white matter. Transplanted ENSC had a preferential distribution around the dorsal spinal cord, with few cells located ventrally. (D) In longitudinal sections GFP + cells formed bridging connections (arrow) between the anterior and posterior segments of the injured spinal cord. GM, grey matter; WM, white matter. Scale bars: (A, B) 1 mm, (C, D) 500 μm.