Table 3.
Primers used for PCR
| Probe target | Primer sequence | Product size | Tm |
|---|---|---|---|
| TuJ1 | F: GCCCCGACAACTTCATTTT | 138 | 63.8 |
| R: GCAGTCGCAGTTCTCACACT | 63.4 | ||
| p75 | F: AGGTGATGGTGAAGGAGTGC | 183 | 64.2 |
| R: GACGGTGGTGACAATGTCTG | 64.3 | ||
| S100 | F: AGTACTCCGGGAAGGAAGGA | 144 | 63.8 |
| R: GTCCAGTGCCTCCATGACTT | 64.2 | ||
| Sox10 | F: AGCCTTCACAGGGTTTGCT | 135 | 63.8 |
| R: GAGAGGCAGTGGTGGTCTTC | 63.9 | ||
| ChAT | F: AATGCCAGAACCAGAGCACA | 189 | 65.8 |
| R: TCAGTCGTCAGCAAGCCAAT | 66.0 | ||
| GAD | F: GACATCCACCGCTAACACCA | 131 | 66.0 |
| R: CGCCATCTTTATTCGACCATCC | 68.3 | ||
| GLS1 | F: CTTACTCAAGCTTTCAGGAGGAA | 194 | 62.6 |
| R: TGCCCATCCACTGTGCAAA | 69.0 | ||
| nNOS | F: ATGCTCAACTACCGCCTCAC | 117 | 64.2 |
| R: AATGGCCCTCTTCTTGGTGG | 67.3 | ||
| TPH1 | F: GTGCTGATGTACGGGTCTGA | 112 | 69.3 |
| R: AGTTCATAGCCAGGTCTGCA | 62.5 | ||
| TPH2 | F: CTCTATCCCACCCACGCTTG | 159 | 66.7 |
| R: AACCGGTCTCACTGTGAAGC | 64.3 |