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Effect of Hyp on apoptotic cells in fibrillar Aβ1–42-induced bEnd.3 cells (fluorescence microscopy, 200× magnification).
Apoptotic nuclei stained by TUNEL present green fluorescence and even nuclear fragmentation (red arrows). All nuclei are stained by DAPI with dark blue fluorescence. The experiment was conducted in triplicate. Aβ1–42: Amyloid beta 1–42; Hyp: hyperoside; TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling; DAPI: 4′,6-diamidino-2-phenylindole.
