Figure 4.

Hyp prevents Aβ_1–42-induced apoptosis in bEnd.3 cells via inhibiting mitochondrial, endoplasmic reticulum, and death receptor signaling pathways.

Results of western blot assay showed that ratios of Bax/Bcl-2, cleaved caspase-9/caspase-9, cleaved caspase-12/caspase-12, cleaved caspase-8/caspase-8, and cleaved caspase-3/caspase-3 were increased, while cytochrome c protein was reduced in Aβ_1–42-induced apoptosis in bEnd.3 cells. Equal amount of protein loading was confirmed by actin levels. All results are represented as the mean ± SEM (one-way analysis of variance followed by Tukey's multiple comparison post hoc test). The experiment was conducted in triplicate. ##P < 0.01, vs. control group; **P < 0.01, vs. 20 µM Aβ_1–42 group. Aβ_1–42: Amyloid beta 1–42; Hyp: hyperoside.