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The expression of cytokines by CXCR5+ CD8+ memory T Cells from tonsils, lymph nodes and PBMCs. The mononuclear cells from tonsils, lymph nodes and blood were cultured with or without PMA plus ionomycin in the presence of BFA for 6 h. FACS analysis of IL-21, IL-4, and IFN-γ expression in CXCR5+ and CXCR5− CD8+ memory T Cells (A–C). Data represent mean ± SD, and compared with two-tailed unpaired t-test (D, n = 11). *P < 0.05, **P < 0.01, and ***P < 0.001. ns, no significance.
