Table 1.

Formulation compositions evaluated using freeze-thaw-stability studies. The potency values were obtained for untreated control samples, freeze-thawed samples and freeze-thawed samples incubated at 25^oC for one week (freeze-thawed-stability). LOQ represent Limit of Quantification i.e. virus not detected since viral titer losses exceeded limit of quantification of the assay. Error represent SD propagated from six replicates each of samples used for calculation.

Formulation	Hepes (mM)	NaCl (mM)	Ratio Sucrose (g/100 mL)/ PVP K-25 (g/100 mL)	L- Arginine (mM)	Glycine (mM)	CaCl2.2H2O (mM)	ZnCl2 (mM)	Tween-20 (%w/v)	Titer loss in freeze-thawed samples (Log10 IU/mL)	Titer loss in freeze-thawed-stability samples(Log10 IU/mL)
HSRV00	10	50	1.2/4.8	100	0	2	0	0.02	0.06 ± 0.14	0.90 ± 0.13
HSRV01	25	25	3/3	50	50	1	1	0.01	0.00 ± 0.10	1.20 ± 0.31
HSRV02	10	0	1.2/4.8	10	0	0	0	0	0.07 ± 0.10	<LOQ
HSRV03	10	50	4/2	10	0	2	2	0	<LOQ	<LOQ
HSRV04	50	0	4/2	100	0	2	0	0	0.00 ± 0.10	0.50 ± 0.21
HSRV05	50	50	1.2/4.8	10	100	2	0	0	0.00 ± 0.14	0.50 ± 0.09
HSRV06	10	0	1.2/4.8	100	100	2	2	0	0.12 ± 0.19	<LOQ
HSRV07	50	50	4/2	10	0	0	0	0.02	0.00 ± 0.09	<LOQ
HSRV08	50	50	1.2/4.8	100	0	0	2	0	0.15 ± 0.24	<LOD
HSRV09	50	0	1.2/4.8	10	0	2	2	0.02	0.19 ± 0.11	1.80 ± 0.28
HSRV10	10	0	4/2	10	100	2	0	0.02	0.06 ± 0.09	1.30 ± 0.30
HSRV11	50	50	4/2	100	100	2	2	0.02	0.03 ± 0.08	<LOQ
HSRV12	50	0	4/2	10	100	0	2	0	0.06 ± 0.21	<LOQ
HSRV13	10	50	4/2	100	100	0	0	0	0.05 ± 0.14	0.20 ± 0.11
HSRV14	50	0	1.2/4.8	100	100	0	0	0.02	0.09 ± 0.10	<LOQ
HSRV15	10	50	1.2/4.8	10	100	0	2	0.02	0.10 ± 0.19	0.50 ± 0.08
HSRV16	25	25	3/3	50	50	1	1	0.01	0.26 ± 0.13	<LOQ
HSRV17	10	0	4/2	100	0	0	2	0.02	0.00 ± 0.10	<LOQ
HSRV18	25	25	3/3	50	50	1	1	0.01	0.15 ± 0.17	<LOQ