Table 2.
Formulations prepared by varying excipients. The stabilizing buffer solutions were coded as HSRV04B, HSRV04C, HSRV04D and HSRV04E. The formulations were prepared by mixing 85% of stabilizing buffer by volume, adjusted to have concentrations indicated, with 15% by volume of mixed rotavirus bulk. The Tg’ is shown as mean ± SD of three samples.
| Ingredients | HSRV04 | HSRV04B | HSRV04C | HSRV04D | HSRV04E |
|---|---|---|---|---|---|
| HEPES (mM) | 50 | 50 | 50 | 50 | 50 |
| Sucrose (%) | 4.0 | 4.0 | 4.0 | 6.0 | 4.0 |
| PVP K-25 (%) | 2.0 | 4.0 | 6.0 | 4.0 | 0 |
| PVP K-40 (%) | 0 | 0 | 0 | 0 | 2.0 |
| L-Arginine (mM) | 100 | 100 | 100 | 100 | 100 |
| CaCl2.2H2O (mM) | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 |
| Tg’ (oC) | −31.6 ± 0.3 | −29.0 ± 0.8 | −28.7 ± 0.4 | −29.8 ± 0.2 | −31.5 ± 0.0 |