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(A, B) ST2 cells (A) and human MSCs (B) were pre-treated with or without TGFβ (10 ng/mL) for 72 h and then stimulated with PDGF-BB (20 ng/mL) for 15 min or 1 h. Cell lysates were subjected to western blotting with anti-p-PDGFRβ, anti-PDGFRβ, and anti-GAPDH antibodies. (C) A proposed scheme of how the functions of PDGFRβ change during differentiation into cancer-associated fibroblasts.
