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. 2018 Sep 25;9(75):34132–34141. doi: 10.18632/oncotarget.26139

Figure 1. Establishment of 2D3 cell line.

Figure 1

(A) Schema of 2D3 cells. The transduction of TCRs into 2D3 cells recruits CD3 onto the cell surface, and appropriate TCR signaling induced by antigen recognition activates the NFAT-GFP reporter gene, followed by GFP production. NFATx3, NFAT-binding sites x3; IL-2 mini pro, IL-2 minimal promoter. (B) Expression of CD3 and CD8 in Jurkat-76, J76.7, and 2D3 cells. Representative contour plots are shown. (C) GFP expression in 2D3 cells after PMA/Ionomycin stimulation. Representative contour plots are shown.