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. 2018 Sep 25;9(75):34038–34055. doi: 10.18632/oncotarget.25954

Figure 3. Impact of conditioned media (CM) obtained from cisplatin-treated lung cancer cells on the migration, viability, and tube formation of HUVECs.

Figure 3

(A) and (C), Migration (black bars, modified Boyden chamber assay), viability (white bars, WST-1 assay), and tube formation (grey bars) of HUVECs following resuspension in serum-free vehicle-containing DMEM (unconditioned media [UCM]) or CM from vehicle- or cisplatin-treated A549 (A) or H358 (C) cells. A549 and H358 cells were incubated for 48 h with vehicle or 0.01 µM to 1 µM cisplatin in serum-free DMEM prior to CM being collected for the generation of HUVEC suspensions. Migration and viability of HUVECs were measured following a 24-h incubation of HUVECs suspended in the indicated CM. Tube formation was determined following a 2-h incubation of HUVECs suspended in the indicated CM. (B), Phase contrast images of tube formation on the Matrigel layer following a 2-h incubation with the respective treatments. Images are a representative view of HUVECs tested in (A). Percentage of control represents comparison with UCM (set as 100%), i.e., vehicle-treated HUVECs in serum-free DMEM in the absence of cisplatin and cancer cells. Values are the mean + SEM of n = 6 (A, migration) or n = 3 (A, viability, tube formation, C). **p < 0.01, ***p < 0.001 vs. vehicle-containing UCM. ## p < 0.01, ### p < 0.001 vs. CM of vehicle-treated A549 or H358 cells, one-way ANOVA plus a post-hoc Bonferroni test.