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. 2018 Oct 8;10:4311–4323. doi: 10.2147/CMAR.S173496

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© 2018 Zhang and Zhang. This work is published and licensed by Dove Medical Press Limited

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K562 cell apoptosis upon knockdown of NUDT21.

Notes: (A) The activities of Caspase3/7 were determined by Caspase-Glo 3/7 assays in K562 cells (1.5×10⁴) after transfection of shRNA control or NUDT21 shRNA. (B) The percentage of annexin V-positive revealed apoptosis. Percentages of cells undergoing apoptosis in different groups are shown. (C and D) Bax and Bcl-2 protein were determined by Western blot analysis in K562 cells after transfection of shRNA control or NUDT21 shRNA. Data are expressed as mean ± SD for duplicate experiments. ^**P<0.01; ^***P<0.001.

Abbreviation: PI, propidium iodide.