Table 1.
Coordinates of human intracellular signaling array
| Target | Phosphorylation site | Modification | P-value | Up/down | |
|---|---|---|---|---|---|
| 1 | Positive control | N/A | N/A | \ | \ |
| 2 | Negative control | N/A | N/A | \ | \ |
| 3 | p-ERK1/2 | Thr202/Try204 | Phosphorylation | 0.000 | –0.207 |
| 4 | p-Stat1 | Tyr701 | Phosphorylation | 0.262 | 0.105 |
| 5 | p-Stat3 | Tyr705 | Phosphorylation | 0.001 | 0.223 |
| 6 | p-Akt(Thr308) | Thr308 | Phosphorylation | 0.357 | 0.090 |
| 7 | p-Akt(Ser473) | Ser473 | Phosphorylation | 0.790 | 0.041 |
| 8 | p-AMPKa | Thr172 | Phosphorylation | 0.000 | 0.304 |
| 9 | p-S6 Ribosomal Protein | Ser235/236 | Phosphorylation | 0.526 | –0.075 |
| 10 | p-mTOR | Ser2448 | Phosphorylation | 0.004 | 0.351 |
| 11 | p-HSP27 | Ser78 | Phosphorylation | 0.240 | 0.136 |
| 12 | p-Bad | Ser112 | Phosphorylation | 0.020 | 0.212 |
| 13 | p-p70 S6 Kinase | Thr389 | Phosphorylation | 0.799 | 0.036 |
| 14 | p-PRAS40 | Thr246 | Phosphorylation | 0.004 | 0.125 |
| 15 | p-p53 | Ser15 | Phosphorylation | 0.001 | 0.467 |
| 16 | p-p38 | Thr180/Tyr182 | Phosphorylation | 0.191 | 0.083 |
| 17 | p-SAPK/JNK | Thr183/Tyr185 | Phosphorylation | 0.692 | 0.024 |
| 18 | Cleaved PARP | Asp214 | Cleavage | 0.023 | 0.124 |
| 19 | Cleaved Caspase3 | Asp175 | Cleavage | 0.000 | 0.492 |
| 20 | p-GSK-3β | Ser9 | Phosphorylation | 0.015 | 0.050 |
Notes: The phosphorylation of ERK1/2, which is the effector protein of the MPKA/ERK pathway, was reduced significantly. Meanwhile, the cleaved Caspase3 raised in the shNUDT21 group compared with the shRNA control group (P<0.001).