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Primer sequences used for RT-PCR gene expression analysis
| Genes | 5′-3′ | Primers | Production size (bp) |
|---|---|---|---|
| ALP | Forward | CCTGGACCTCATCAGCATTT | 279 |
| Reverse | AGGGAAGGGTCAGTCAGGTT | ||
| Runx2 | Forward | TCTCTGACCGCCTCAGTGATT | 171 |
| Reverse | TGTGTCTGCCTGGGATCTGTA | ||
| BMP-2 | Forward | GAAGCCAGGTGTCTCCAAGAG | 142 |
| Reverse | GTGGATGTCCTTTACCGTCGT | ||
| OPN | Forward | GGAGTCCGATGAGGCTATCAA | 208 |
| Reverse | TCCGACTGCTCAGTGCTCTC | ||
| Col 1 | Forward | TGGATGGCTGCACGAGT | 177 |
| Reverse | TTGGGATGGAGGGAGTTTA |
