Skip to main content
. 2018 Jul 20;69(21):5059–5075. doi: 10.1093/jxb/ery269

Fig. 5.

Fig. 5.

GhHUB2 interacts with GhKNL1 and co-localizes with GhKNL1 in the nucleus. (A) GhHUB2 interacts with full-length GhKNL1 in yeast. (B) KNOX1 and KNOX2 domains in GhKNL1 mediate the interaction of GhHUB2 and GhKNL1. (C) GhHUB2 and GhKNL1 co-localize in the nucleus. Scale bars are 10 µm. (D) GhHUB2 interacts with GhKNL1 in a split firefly luciferase complementation assay. GhHUB2 fused to the C-terminus of LUC (cLUC-GhHUB2) was co-expressed with a GhKNL1-fused N-terminus of LUC (GhKNL1-nLUC) in Nicotiana benthamiana leaves. Images were taken 3 d after infiltration. The RING domain E3 ligase GhSINA and the homeodomain transcription factor GhHOX3 were used as negative controls. (E) GhHUB2 interacts with GhKNL1 in a co-immunoprecipitation (Co-IP) assay. FLAG-GhHUB2 and MYC-GhKNL1-GFP were co-expressed in N. benthamiana leaves, and the 26S proteasome inhibitor MG132 (100 µM) was infiltrated 14 h before sample collection. Co-IP was carried out with anti-FLAG agarose from the total isolated proteins, and immunoblotting analysis was performed with anti-FLAG and anti-MYC antibodies. Full size images are shown in Supplementary Fig. S6.