Fig. 7.

GhHUB2 promotes GhKNL1 degradation and releases GhKNL1-suppressed genes in cotton fibers. (A) qRT-PCR analysis of GhHUB2 expression in fibers at 6–20 d post-anthesis (DPA). (B) qRT-PCR analysis showing that the RNA transcription level of GhKNL1 did not change between transgenic lines and the CCRI24 wild-type (WT. (C) GhHUB2 promotes the degradation of GhKNL1 in developing fibers. The abundance of GhKNL1 protein was detected among fiber-extracted nuclear proteins using anti-GhKNL1 antibodies at 6–20 DPA. Cotton Histone3 detected by anti-H3 antibodies was used as a loading control. (D) qRT-PCR analysis of GhKNL1-triggered genes in transgenic cotton fibers. Histone3 was used as the internal control. Data are means (±SE) of triplicate experiments. Significant differences compared with the WT were determined using Student’s t-test; *P<0.05, **P<0.01.