Fig. 7.

16γzf does not form protein bodies (PBs) but forms electron-dense structures in highly enlarged endoplasmic reticulum (ER) lumen. Leaves from 6-week-old transgenic Arabidopsis plants accumulating 16γzf in high (A, B, D, E) or low (C) amounts were analysed. (A, C) Ultrathin sections post-fixed with osmium. (B, D, E) Immunolabelling with anti-FLAG antibody (B, D) or irrelevant antibody as a negative control (E), and secondary 15-nm gold-conjugated goat anti-rabbit serum. The insets in (A–C) show magnifications to better illustrate the ribosomes attached on the cytosolic side of the ER membrane (arrows) and the electron-dense convoluted structures within the ER lumen. Ch, chloroplasts; M, mitochondria; V, vacuole. Scale bars in (A, D) and all insets are 200 nm; bars in (B, C, E) are 500 nm.