Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 12;4(10):eaau1338. doi: 10.1126/sciadv.aau1338

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).

This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 4 — (A) Reference image of a fluorescent micropattern without any scattering sample. (B) Image obtained by conventional TF microscopy (i.e., under uniform wide-field TF illumination with wide-field detection in epifluorescence configuration) through 400 μm of fixed rat brain tissue. (C and D) Reconstructed images obtained with TRAFIX through 200 and 400 μm of rat brain tissue, respectively. The two retrieved images were reconstructed using a full Hadamard basis containing 1024 patterns. Camera binning was set to 64 × 64, and exposure time values were (C) 0.2 s and (D) 1 s. Small intensity variations in the reconstructed images arise from inhomogeneities in the fluorescent micropattern originated in the imprinting process. Larger intensity variations are due to inhomogeneities in light transmission through the highly anisotropic scattering medium. This also applies to figs. S7, S9, S10, and S12. The SBR is shown for all reconstructed images. Scale bar, 10 μm.