Skip to main content
. 2018 Oct 12;7:e38309. doi: 10.7554/eLife.38309

Figure 3. Disrupted neural pathways in the Srd5a3 mutant developing cerebellum.

(A, upper panel) Pie chart representing the total proteomics analysis in P7 cerebellum (n = 4 for each genotype). 97 proteins were detected as differentially abundant proteins (DAP) in the mutant samples (q-value <0.05). (A, lower panel) Distribution of unchanged proteins (white box), increased DAP (green box) and decreased DAP (red box) into ER stress-related (GO:0034976) or N-glycoproteins (Reference glycoproteomic database, see Materials andmethod) categories. A significant enrichment was detected within the decreased DAP for N-glycoproteins (n = 8, Fisher exact test, p-value=0.0012). (B) Over-representation analysis on DAP performed with ConsensusPathDB using the 1982 detected proteins as background. All pathways with p-value<0.01 are represented. N-glycoproteins are indicated in green. (C) Average multiplicity in the unchanged and the decreased N-glycoproteins groups. (D) Enrichment for different glycoprotein categories among the decreased N-glycoproteins. Multiplicity represents groups of proteins with more than one (≥2), two (≥3) or three (≥4) N-glycosylation sites. Multiplicity of ≥1 represents all of the decreased N-glycoproteins and is set to one (no enrichment). There is a significant 2.4 fold-change enrichment for glycoproteins with four or more N-glycans among the decreased N-glycoproteins (Fischer exact test, p-value=0.0378).

Figure 3.

Figure 3—figure supplement 1. Extended total proteomic analysis of the unfolded protein response (UPR).

Figure 3—figure supplement 1.

(A) Principal component analysis (PCA) based on the abundance profiles of the 1982 detected proteins. (B) Transcriptomic analysis of UPR-related mRNAs in the control (n = 4) and mutant (n = 4) cerebellum. (C) Immunocytochemistry against BiP in MEFs treated with tunicamycin (Tm) for 24 hr to induce UPR activation and BiP over-expression (positive control) in mouse cells. Scale bar 25 μm. (D) Immunohistochemistry against BiP and NeuN in the P21 mouse cerebellum. The arrowhead points to ectopic GCs. Scale bar 50 μm. Student t-test was used for statistics. Results are presented as mean ± s.d.