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. 2018 Jan 17;27(7):1136–1149. doi: 10.1093/hmg/ddy022

Figure 1.

Figure 1.

CHEK1 is required for the proliferation of prospermatogonia. (A) Western blotting of testicular lysate obtained from wild-type mice at 8, 11, 14 and 17 dpp, and from Chek1ctrl and Ddx4-Cre-ERT2: Chek1cKO mice at 31 dpp (tamoxifen injections were at 3 weeks of age, or 21 dpp, and testes were harvested 10 days later). Asterisks indicate the positions of non-specific bands. Anti-CHEK1 antibody (Sigma #C9358) was used. (B) Schematic for Chek1 deletion by Ddx4-Cre. (C) Picture of Chek1ctrl and Chek1cKO testes at 54 dpp. Scale bar: 1 cm. (D) Immunostaining of WT1 and PLZF on testicular sections at 24 dpp. Scale bars: 100 μm. (E) Immunostaining of SCML2 on testicular sections at 3 and 5 dpp. White arrowheads indicate SCML2-positive cells in the Chek1cKO. Nuclei were counterstained with DAPI. Scale bars: 100 μm.