FIGURE 2.

Four to 6 colonic and ileal biopsies are sufficient for robust analyses of immune cell subsets using mass cytometry. A, Yields of live CD45^positive events, derived from the indicated number of colon and ileal biopsies, as acquired by CyTOF. Live CD45^positive events were identified by gating on nucleated cells, followed by viability staining and CD45 expression. Data points represent paired individual donors, shown as mean ± SD. B, t-SNE analysis of live CD45^positive events derived from 4 colonic (left) or ileal (right) biopsies. Color overlays highlight each immune lineage. t-SNE plots display data representative of 4 individual donors. C, Biaxial plots displaying the phenotype of each major population identified by t-SNE analysis in (B). Plots are representative of 4 individual donors.