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. 2018 Jun 8;24(11):2366–2376. doi: 10.1093/ibd/izy214

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FIGURE 4. — Cryopreservation maintains the integrity of stained samples for delayed acquisition. Percentage live cells (A) and total CD45^positive cells (B) acquired after isolation from either colonic biopsies or peripheral blood, and acquired immediately after staining (Fresh) or after cryopreservation (Cryo) at –80°C for up to 2 months. Data points represent individual donors. Percentage cell recovery was calculated as yield derived from cryopreserved cells as a proportion of yield in fresh samples, in paired sample sets. C, Representative biaxial plots of expression of indicated lineage markers, from fresh or cryopreserved samples. F, Median signal intensity (MSI) of major lineage markers in fresh or cryopreserved samples after staining. Mean ± SD of 5 individual donors, analyzed using the Wilcoxon test.