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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Clin Neurosci. 2018 Jul 21;56:163–168. doi: 10.1016/j.jocn.2018.06.005

Figure 1. Saline as a carrier for cell-based CED fails to deliver CAR T cells.

Figure 1

MedFusion 3010a syringe clinical pumps were loaded with a 20cc syringe containing a saline solution of 3.8 × 107 T cells/ml. This was connected to a 20 cm long coiled silicon extension tube. These pumps were placed either horizontally (A) or vertically (B) and the infusion rate was adjusted to 500 µl/hr. All infusion procedures were performed for 5 hours at room temperature to replicate a clinical environment. Collected infusates after CED were transferred to a 1.7 ml eppendorf tube and counted using trypan blue exclusion method. The Expected value indicates the theoretical yield of 100% delivery efficiency. C) Comparison of the efficiency of CAR T cell delivery (yield) from horizontally and vertically positioned infusion pumps. CAR T cell delivery yield (%) was calculated: (CAR T cells counted in infusate per ml per hour/Expected (3.8 × 107 T cells/ml per hour)) × 100.