Figure 2. Hyaluronic acid based LVHydrogel increases the CAR T cell delivery rate of CED.

MedFusion 3010a syringe clinical pumps were loaded with a 20cc syringe containing either a saline solution or LVHydrogel containing 1.2×10⁷–1.7×10⁷ CAR T cells/ml, and submitted to a 5 hour infusion procedure at room temperature. Collected infusates after CED were transferred to a 1.7 ml eppendorf tube and counted using trypan blue exclusion method. A) The efficiency of CAR T cell delivery (yield) in different carriers was calculated: CAR T cell delivery yield (%) was calculated: (CAR T cells counted in infusate per ml per hour/Expected value assuming 100% delivery efficiency. The Expected value was the concentration of CAR T cells at the time of infusion per hour)) × 100. B) Viability of the CAR T cells present in infusate was determined using the trypan blue exclusion method. Percent viable cells was calculated: (Live cells/(Live+Dead cells)) × 100. Statistical analysis was performed using the Bonferroni-corrected t-test. Significance was determined at the level of *p < 0.05.