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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Clin Neurosci. 2018 Jul 21;56:163–168. doi: 10.1016/j.jocn.2018.06.005

Figure 3. Effective Migration Assay of Human CAR T cells in LVHydrogel.

Figure 3

The lower chambers of 24-well transwell plates were loaded with media containing the human chemokine CXCL10 at the indicated concentrations: 1 ng/ml, 10ng/ml, 100 ng/ml, 500 ng/ml and 1000 ng/ml. The upper chamber of each well was seeded with serum starved 6 × 105 CAR T cells in either saline or LVHydrogel carriers. Transwell plates were then incubated at 37°C for 12 hours. Plates were then removed from the incubator and the cells in the lower chamber of the transwell were counted in a hemocytometer using trypan blue exclusion counting method. Percentage of migration by CAR T cells was calculated by the following formula: CAR T cell migration (%) = ((Total number of cells that migrated to lower chamber)/(Total number of cells loaded in upper chamber)) × 100. Statistical analysis was performed using the quadratic interaction term between log-CXCL concentration and group in the regression model. Significance was determined at the level of *p < 0.05.