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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Biotechnol Bioeng. 2018 Jul 20;115(10):2394–2404. doi: 10.1002/bit.26772

Table II.

Active mutants from multi-mutant saturation mutagenesis library

Sequences of active variants from the multi-mutant initial screening with at least two mutations are listed. Blank cells are wild-type amino acids. The activities of these variants against Salmonella enterica serovar enteritidis (SE) are listed.

Position
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 Activity against SE†
Wild-type MccJ25
G G A G H V P E Y F V G I G T P I S F Y G +++++
Active multi-mutant
A Y ++
F T +
F Y ++
L Y +
S A +
N F ++
N M +
N A +
N S +
T F +
T A +++
T T ++++
T Y +++
S F ++++
S T +
S N +
A N Y +
A T F +
F T A +
T M Y +
S M Y +
S N Y +
S T Y +
A M L T +
†

Activity against SE was determined by zone-of-inhibition size for each mutant and indicated in one of five bins <20%, <40%, <60%, <80%, and 100% of wild-type MccJ25 indicated by +, ++, +++, ++++, and +++++, respectively.