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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: J Cell Physiol. 2018 Jun 26;233(12):9563–9574. doi: 10.1002/jcp.26859

Figure 6. Depletion of BMAL1 or CLOCK alters UVB-induced DNA damage responses and induces terminal differentiation in HKCs.

Figure 6

(A) HKCs were transfected with control siRNA or siRNAs against BMAL1 or CLOCK and exposed to 5 mJ/cm2 UVB radiation. Cells were harvested at 6 h or 24 h post UVB irradiation for western blot analysis of γ–H2AX. (B) Protein lysates collected at 24 h post UVB irradiation were analyzed for p-p53 and total p53 protein expressions. (C) Cells were collected at 48 h or 72 h after siRNAs transfection (without UVB irradiation) and subjected to RT-PCR analysis or western blot analysis of keratin 1 (KRT1) or keratin 10 (KRT10). The mRNA level of each gene was normalized to 36B4, and presented as mean-fold over control ± S.E.M. *, p < 0.05, ***, p < 0.001, ****, p < 0.0001, N=3. Relative protein levels were quantified by densitometry scanning with ImageJ and normalized to α–tubulin. Data were presented as mean-fold over control ± S.E.M. *, p < 0.05, **, p < 0.01, ***, p < 0.001, ns, not significant, N=3.