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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Glia. 2018 Jul 25;66(9):2024–2039. doi: 10.1002/glia.23455

Figure 4. Astrocyte glutathione metabolic up-regulation is dependent on neuronal activity.

Figure 4

A) qRT-PCR results for glutathione metabolic genes in astrocytes co-cultured with neurons relative to naïve astrocytes. Cultures were untreated or treated with the indicated pharmacological blockers of neuronal synaptic activity (n=3). * p < 0.048, ** p < 0.01, *** p < 0.001 relative to naïve astrocytes using one-way t-test; Δ p < 0.048, ΔΔ p < 0.01, ΔΔΔ p < 0.001 relative to untreated co-cultured astrocytes using Kruskal-Wallis one-way analysis of variance. Error bars show 95% confidence intervals. B) Box plots of Thioltracker (as in Fig. 3E) comparing naïve astrocytes and astrocytes co-cultured with neurons in the presence or absence of inhibitors of neuronal activity TTX and CNQX (n=3 replicates). ** p < 0.01, **** p < 0.0001 between indicated conditions using one-way analysis of variance.