Skip to main content
. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Glia. 2018 Jul 25;66(9):2024–2039. doi: 10.1002/glia.23455

Figure 5. A set of astrocyte glutathione genes is up-regulated in response to secreted glutamate from neuronal cultures.

Figure 5

A) qRT-PCR results for glutathione metabolic genes in naïve astrocytes exposed to conditioned media (NCM) from neurons grown in isolation for 10DIV, with or without inhibitors of metabotropic glutamate receptors (mGluRs) LY341495 and MPEP (n=4). * p < 0.048, ** p < 0.01, *** p < 0.001 relative to control media not incubated with neurons using one-way t-test; Δ p < 0.048, ΔΔ p < 0.01 relative to untreated astrocytes in NCM using Kruskal-Wallis one-way analysis of variance. B) qRT-PCR results for glutathione metabolic genes in naïve astrocytes exposed to glutamate or the mGluR agonist (S)-3,5-DHPG (n=3). * p < 0.048, ** p < 0.01, *** p< 0.001 relative to untreated astrocytes using one-way t-test. C) qRT-PCR results for glutathione metabolic genes in astrocytes co-cultured with neurons relative to naïve astrocytes. Cultures were treated with mGluR antagonists LY341495 and MPEP singly or in combination (n=3). * p < 0.048, ** p < 0.01, *** p < 0.001 relative to naïve astrocytes using one-way t-test; Δ p < 0.048, ΔΔ p < 0.01 relative to untreated co-cultured astrocytes using Kruskal-Wallis one-way analysis of variance. Error bars show 95% confidence intervals in all panels.