Table 1. Data collection and refinement statistics.

Sequence	5’-TGAGGGTGGGTAGGGTGGGTAA-3’
Data Collection
Space group	P 21 21 2
Unit cell dimensions (a, b, c) (Å) α, β, γ (°)	65.7 69.8 33.0 90 90 90
Wavelength (Å)	0.9793
Resolution (Å)*	34.91–2.35 (2.43–2.35)
Total reflections*	85280 (8398)
Unique reflections*	6774 (652)
Multiplicity*	12.6 (12.9)
Completeness (%)*	99.4% (99.7%)
I/σ*	33.9 (4.4)
Wilson B-factor (Å2)	58.8
Rmeas†*	0.042 (0.605)
CC1/2‡*	1.000 (0.966)
Refinement
Resolution (Å)*	34.91–2.35 (2.53–2.35)
Rwork§*	0.220 (0.319)
Rfree§*	0.245 (0.322)
Macromolecules	2
DNA Residues	44
Total Atoms	956
Potassium ions	6
Waters	16
Average Overall B-factor (Å2)	80.2
Average B-factor DNA Residues (Å2)	80.7
Average B-factor Potassium Ions (Å2)	53.1
Average B-factor Waters (Å2)	59.7
RMS (bonds) (Å)	0.009
RMS (angles) (°)	1.08
TLS groups	2
PDB ID	6AU4

*Statistics in parentheticals are for the high-resolution shell

^† $R_{meas}=\frac{{\sum }_{hkl}\sqrt{\frac{n}{n-1}}{\sum }_{i=1}^n{|I}_i\left(hkl\right)-\overline{I}\left(hkl\right)|}{{\sum }_{hkl}{\sum }_{i=1}^n{I}_i\left(hkl\right)}$, where I_i(hkl) is the ith observation of the intensity of the reflection hkl and n is the multiplicity.

^§ $R_{work}=\frac{{\sum }_{hkl}|{|F}_{obs}|-{|F}_{calc}||}{{\sum }_{hkl}{|F}_{obs}|}$, where F_obs and F_calc are the observed and calculated structure-factor amplitudes for each reflection hkl. R_free was calculated with 10% of the diffraction data that were selected randomly and excluded from refinement.

^‡CC_1/2 is the intra-dataset Pearson correlation coefficient (CC) calculated by randomly assigning the experimental reflection data to two half-datasets (x, y). $CC=\frac{{\sum }_{i=1}^n\left(x_i-\overline{x}\right)-(y_i-\overline{y})}{\sqrt{{{\sum }_{i=1}^n\left(x_i-\overline{x}\right)}^2}\sqrt{{{\sum }_{i=1}^n(y_i-\overline{y})}^2}}$